Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists01:23

Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists

Serotonin, a crucial neurotransmitter synthesized by enterochromaffin cells, plays a cardinal role in regulating gastrointestinal (GI) motility. With over 90% of the body's total serotonin in the GI tract, its influence on digestive processes is profound. Serotonin is swiftly released upon various stimuli, such as food boluses or certain drugs, triggering intrinsic sensory neurons in the myenteric plexus and extrinsic vagal and spinal sensory neurons. This leads to the activation of the...
Fermentation01:29

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Antidepressant Drugs: MAOIs and Other Agents01:23

Antidepressant Drugs: MAOIs and Other Agents

Atypical antidepressants, including bupropion (Wellbutrin), mirtazapine (Remeron), nefazodone (Serzone), trazodone (Desyrel), and vilazodone (Viibryd), offer unique mechanisms of action. Bupropion weakly inhibits dopamine and norepinephrine reuptake, aiding depression treatment and smoking cessation, with a low risk of sexual dysfunction. Mirtazapine enhances serotonin and norepinephrine neurotransmission, leading to sedation, increased appetite, and weight gain. As a result, it helps treat...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Primary revision with replasty of the anterior cruciate ligament].

Operative Orthopadie und Traumatologie·2019
Same author

[Arthroscopic suprapectoral tenodesis of the long head of the biceps tendon].

Operative Orthopadie und Traumatologie·2018
Same author

Experiences with Osteoligamentoplasty According to Weiss for the Treatment of Scapholunate Dissociation.

Journal of investigative surgery : the official journal of the Academy of Surgical Research·2017
Same author

[Partial joint replacement of the knee : A sophisticated technique].

Operative Orthopadie und Traumatologie·2017
Same author

[Arthroscopic refixation of acute proximal anterior cruciate ligament rupture using suture anchors].

Operative Orthopadie und Traumatologie·2016
Same author

[Suturing and refixation of the medial collateral ligament in severe acute medial instability of the knee].

Operative Orthopadie und Traumatologie·2015

Related Experiment Video

Updated: Jun 19, 2026

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
08:49

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Published on: September 23, 2015

SEROTOXIN : STUDIES ON FERMENT ACTION. XIV.

J W Jobling1, W Petersen

  • 1Department of Pathology of the College of Physicians and Surgeons, Columbia University, New York.

The Journal of Experimental Medicine
|October 30, 2009
PubMed
Summary

Removing protective lipoids from serum creates a toxic substance, serotoxin, which causes coagulation issues and protein exposure. Adding back lipoids or unsaturated soaps neutralizes this toxicity, restoring serum safety.

Area of Science:

  • Biochemistry
  • Immunology
  • Physiology

Background:

  • Serum, a vital biological fluid, contains protective lipoids essential for its stability.
  • The removal of these lipoids can render serum toxic, a phenomenon not fully understood.
  • Understanding serum toxicity is crucial for transfusion safety and therapeutic applications.

Purpose of the Study:

  • To investigate the toxic effects of lipoid-depleted serum (serotoxin).
  • To identify the factors contributing to serum toxicity.
  • To determine methods for neutralizing serum toxicity.

Main Methods:

  • Lipoid extraction from animal sera.
  • Administration of lipoid-depleted serum to homologous animals.
  • Analysis of coagulation mechanisms, protein exposure, and autolysis.

Related Experiment Videos

Last Updated: Jun 19, 2026

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
08:49

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Published on: September 23, 2015

  • Testing the effects of heat, hirudin, sodium citrate, and lipoid/soap reintroduction.

    • Main Results:

    • Lipoid-depleted serum is toxic, causing intravascular coagulation and exposing native serum proteins.
    • Toxicity is linked to autolysis and the formation of toxic proteoses.
    • Toxicity can be neutralized by reintroducing extracted lipoids or unsaturated soaps.
    • Heating to 70°C reduces toxicity, while boiling proteoses retains toxicity.
    • Sublethal doses cause prostration, hypothermia, and altered antitrypsin levels.

    Conclusions:

    • Serum toxicity is primarily mediated by the absence of protective lipoids.
    • The process involves coagulation alterations, protein denaturation, and autolytic breakdown products.
    • Neutralization is achievable through lipoid restoration or unsaturated fatty acids, highlighting their protective role.