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Updated: Jun 19, 2026
07:49
Measuring Local Anaphylaxis in Mice
Published on: October 14, 2014
THE MECHANISM OF ANAPHYLATOXIN FORMATION : STUDIES ON FERMENT ACTION. XV
1Department of Pathology of the College of Physicians and Surgeons, Columbia University, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Serum antitrypsin, a key lipoid, can be adsorbed by various substances like kaolin and bacteria. Its removal from serum can lead to toxic anaphylatoxins, impacting protein stability.
Area of Science:
- Biochemistry
- Immunology
- Lipid Research
Background:
- Serum antitrypsin is an unsaturated lipoid found in various animal sera.
- The interaction between serum antitrypsin and other substances is crucial for understanding serum stability and toxicity.
Purpose of the Study:
- To investigate the adsorption of serum antitrypsin by different agents.
- To explore the role of serum antitrypsin in the formation of anaphylatoxins.
Main Methods:
- Adsorption of serum antitrypsin from guinea pig, rabbit, and horse serum using kaolin, starch, agar, and bacteria.
- Investigating the effect of diphtheria toxin and cobra venom on serum antitrypsin levels.
- Analyzing the quantitative relationships governing antitrypsin adsorption.
Main Results:
- Unsaturated lipoids (serum antitrypsin) were effectively adsorbed by kaolin, starch, agar, and bacteria.
- Diphtheria toxin and cobra venom reduced serum antitrypsin, suggesting lipoid affinity.
- Partial removal of serum antitrypsin resulted in the formation of toxic anaphylatoxins.
- Adsorption effectiveness varied with the quantity of adsorbing substances, with extreme amounts being least effective.
- Bacteria pre-treated with serum or oils lost their ability to adsorb serum antitrypsin.
Conclusions:
- Serum antitrypsin plays a critical role in preventing serum toxicity.
- The adsorption of serum antitrypsin is a quantifiable process influenced by specific substances and their concentrations.
- Understanding these interactions is vital for fields like immunology and toxicology.
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