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Effects of uric acid in aqueous phase on neutrophil alkaline phosphatase
H A Vergnes1, J Grozdea, A Brisson-Lougarre
1Laboratoire d'Enzymologie du Granulocyte, INSERM, CNRS, Toulouse, France.
Biochimica Et Biophysica Acta
|January 8, 1991
Abstract:
In vitro effects of uric acid (2-6-8 trioxypurine) on purified human neutrophil alkaline phosphatase were studied. A marked activation of the enzyme catalyzed reaction is observed. This activation is dose and pH dependent and not influenced by dialysis. Uric acid can form a stable complex with alkaline phosphatase, whose biochemical characteristics (heat stability, reactivity towards some inhibitors) are significantly modified.