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05:47
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
WANDERING CELLS, ENDOTHELIAL CELLS, AND FIBROBLASTS IN CULTURES FROM HUMAN LYMPH NODES.
1Department of Embryology of Carnegie Institution of Washington and the Department of Pathology of Johns Hopkins Medical School, Baltimore.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Wandering cells from human lymph nodes exhibit active ameboid and phagocytic behavior shortly after incubation. Endothelial cells and fibroblasts appear later, with reduced activity, suggesting a potential origin for wandering cells.
Area of Science:
- Cell Biology
- Immunology
- Histology
Background:
- Human lymph nodes are crucial for immune responses.
- Understanding cell behavior in vitro provides insights into in vivo processes.
- The origin and function of different cell types within lymph nodes are of significant interest.
Purpose of the Study:
- To investigate the migratory patterns and cellular characteristics of cells derived from human lymph node cultures.
- To differentiate and characterize various cell types, including wandering cells, endothelial cells, and fibroblasts, based on their morphology and activity.
- To explore the potential relationship between endothelial cells and wandering cells.
Main Methods:
- Incubation of human lymph node cultures for varying durations (2-48 hours).
- Observation and characterization of cell morphology and behavior (ameboid, phagocytic activity).
- Analysis of cell types appearing in plasma clots over time.
Main Results:
- Wandering cells showed active ameboid and phagocytic properties within 2-3 hours.
- Endothelial cells appeared after 24-48 hours, exhibiting less activity and phagocytosis.
- Fibroblasts were observed after 48 hours, characterized by inactivity.
Conclusions:
- Wandering cells display distinct migratory and functional characteristics early in culture.
- The temporal appearance and differing activities of endothelial cells and fibroblasts suggest distinct roles.
- The findings support the hypothesis that wandering cells may originate from endothelial cells.
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