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WANDERING CELLS, ENDOTHELIAL CELLS, AND FIBROBLASTS IN CULTURES FROM HUMAN LYMPH NODES

W H Lewis1, L T Webster

  • 1Department of Embryology of Carnegie Institution of Washington and the Department of Pathology of Johns Hopkins Medical School, Baltimore.

The Journal of Experimental Medicine
|October 30, 2009
PubMed

Insights

Wandering cells from human lymph nodes exhibit active ameboid and phagocytic behavior shortly after incubation. Endothelial cells and fibroblasts appear later, with reduced activity, suggesting a potential origin for wandering cells.

Area of Science:

  • Cell Biology
  • Immunology
  • Histology

Background:

  • Human lymph nodes are crucial for immune responses.
  • Understanding cell behavior in vitro provides insights into in vivo processes.
  • The origin and function of different cell types within lymph nodes are of significant interest.

Purpose of the Study:

  • To investigate the migratory patterns and cellular characteristics of cells derived from human lymph node cultures.
  • To differentiate and characterize various cell types, including wandering cells, endothelial cells, and fibroblasts, based on their morphology and activity.
  • To explore the potential relationship between endothelial cells and wandering cells.

Main Methods:

  • Incubation of human lymph node cultures for varying durations (2-48 hours).
  • Observation and characterization of cell morphology and behavior (ameboid, phagocytic activity).
  • Analysis of cell types appearing in plasma clots over time.

Main Results:

  • Wandering cells showed active ameboid and phagocytic properties within 2-3 hours.
  • Endothelial cells appeared after 24-48 hours, exhibiting less activity and phagocytosis.
  • Fibroblasts were observed after 48 hours, characterized by inactivity.

Conclusions:

  • Wandering cells display distinct migratory and functional characteristics early in culture.
  • The temporal appearance and differing activities of endothelial cells and fibroblasts suggest distinct roles.
  • The findings support the hypothesis that wandering cells may originate from endothelial cells.

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