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Updated: Jun 19, 2026
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
Published on: January 17, 2014
EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS : VII. FURTHER OBSERVATIONS ON THE
1Laboratories of The Rockefeller Institute for Medical Research.
Abstract:
After artificial cultivation for a period of over 3 years Bacterium pneumosintes has maintained its original morphological and cultural characteristics, when grown in the original medium. Adaptation to a saprophytic existence has been accompanied by a loss of pathogenicity. Our strains now grow readily under strictly anaerobic conditions in a variety of media with peptone broth as a base, enriched with fresh tissue, blood, or by the growth of other bacteria. Surface colonies have been obtained on blood agar plates in. an anaerobic jar. These various methods of cultivation are adapted to special purposes. In broth cultures Bacterium pneumosintes grows in larger forms than in the ascitic fluid-tissue medium, but the identity of the microorganisms is proved by their serological reactions and by reversion to the minute forms on transfer to the original medium.
Insights
Bacterium pneumosintes, after over 3 years of artificial cultivation, retains its characteristics but loses pathogenicity. It can now be cultured under anaerobic conditions, proving its identity through serological reactions.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Bacterium pneumosintes is a bacterium with specific growth requirements.
- Understanding its adaptation to artificial cultivation is crucial for research.
Purpose of the Study:
- To investigate the long-term effects of artificial cultivation on Bacterium pneumosintes.
- To determine if the bacterium loses pathogenicity and adapts to new growth conditions.
Main Methods:
- Artificial cultivation of Bacterium pneumosintes for over 3 years.
- Growth trials in various media under strictly anaerobic conditions.
- Serological testing and morphological analysis for identification.
Main Results:
- The bacterium maintained original morphological and cultural characteristics in its original medium.
- Adaptation to saprophytic existence led to a loss of pathogenicity.
- Successful cultivation under anaerobic conditions in enriched peptone broth and on blood agar plates.
- Morphological variations observed in different media, confirmed by serological reactions.
Conclusions:
- Bacterium pneumosintes can be maintained in culture for extended periods.
- Artificial cultivation leads to a loss of pathogenicity and adaptation to anaerobic environments.
- Serological reactions are key to confirming the identity of cultivated strains.
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