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Related Experiment Video

Updated: Jun 19, 2026

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Published on: March 23, 2014

THE COMPLEMENT FIXATION REACTION AS APPLIED TO LEPROSY.

P A Lewis¹, J D Aronson

¹Henry Phipps Institute of the University of Pennsylvania, Philadelphia.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

Leprosy patient blood serum can fix complement with various acid-fast bacteria antigens. This unique multiple fixation property, especially with Bacillus leprae, shows diagnostic potential for leprosy detection.

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Last Updated: Jun 19, 2026

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Published on: March 23, 2014

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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Published on: July 1, 2011

Area of Science:

Immunology
Microbiology
Infectious Diseases

Background:

The diagnosis of leprosy can be challenging.
Previous diagnostic methods may have limitations.

Purpose of the Study:

To investigate the complement fixation capabilities of leprosy patient serum.
To determine if complement fixation with specific antigens can be a diagnostic marker for leprosy.

Main Methods:

Utilized a novel complement fixation method.
Tested leprosy patient serum against a variety of acid-fast bacterial antigens.
Included control sera from normal individuals and patients with tuberculosis and syphilis.

Main Results:

Leprosy serum demonstrated complement fixation with a wide range of acid-fast bacterial antigens.
Over 93% of leprosy cases showed positive reactions, unlike control groups.
Bacillus leprae antigens (emulsion or extract) yielded the most characteristic fixation in leprosy sera.

Conclusions:

The multiple complement fixation property of leprosy serum is a significant finding.
This property holds diagnostic significance for leprosy.
Further research is needed to confirm its diagnostic utility with complete assurance.