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Related Concept Videos

Leishmaniasis01:30

Leishmaniasis

Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...
Antiprotozoal Agents01:21

Antiprotozoal Agents

Leishmaniasis is a widespread parasitic disease caused by several Leishmania species. It affects millions of people each year and remains a major public health problem in endemic regions. First-line treatment relies on pentavalent antimonials, including meglumine antimoniate and sodium stibogluconate. Even so, how these drugs work has not been fully clear, especially their interaction with parasite-specific biochemical pathways. One key target is trypanothione reductase (TR), an enzyme that...
American Trypanosomiasis01:22

American Trypanosomiasis

Chagas disease, or American trypanosomiasis, is a vector-borne parasitic infection caused by Trypanosoma cruzi, a flagellated protozoan (kinetoplastid) of the family Trypanosomatidae. The disease is endemic in Latin America, although cases are increasingly reported worldwide due to human migration. Transmission most commonly occurs when feces of infected triatomine bugs contaminate bite wounds or mucosal surfaces; additional routes include congenital, transfusional, transplant-related, and oral...

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Related Experiment Video

Updated: Jun 19, 2026

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
12:22

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Published on: December 30, 2012

LEISHMANIA DONOVANI IN THE PERIPHERAL BLOOD.

C W Young1, H M Van Sant

  • 1Department of Medicine of the Peking Union Medical College, Peking, China.

The Journal of Experimental Medicine
|October 30, 2009
PubMed
Summary

Human red cells and serum enhance Leishmania donovani growth, leading to a new blood culture method. Researchers also studied parasite distribution in blood strata and antimony treatment effects on Leishmania donovani cultivability.

Area of Science:

  • Parasitology
  • Hematology
  • Medical Microbiology

Background:

  • Leishmania donovani is the causative agent of visceral leishmaniasis.
  • Understanding parasite-host interactions is crucial for diagnosis and treatment.
  • Previous methods for culturing Leishmania donovani have limitations.

Purpose of the Study:

  • To investigate the influence of human red blood cells and serum on Leishmania donovani growth.
  • To determine the distribution of Leishmania donovan bodies within centrifugalized blood strata.
  • To assess the impact of antimony treatment on the cultivability of Leishmania donovani.

Main Methods:

  • Culturing Leishmania donovani in the presence of human red cells and serum.
  • Centrifugalizing diluted blood to analyze cellular strata.

Related Experiment Videos

Last Updated: Jun 19, 2026

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
12:22

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Published on: December 30, 2012

  • Microscopic examination of blood strata for Leishman-Donovan bodies (extracellular and intracellular).
  • Assessing parasite cultivability after antimony treatment.

    • Main Results:

    • Human red cells and serum were found to support the growth of Leishmania donovani.
    • A novel blood culture method was developed based on these growth factors.
    • Leishman-Donovan bodies were observed in specific strata of centrifugalized blood, both extracellularly and intracellularly.
    • Antimony treatment affected the cultivability of Leishmania donovani.

    Conclusions:

    • Human red cells and serum are essential for Leishmania donovani growth in vitro.
    • The developed blood culture method offers a new approach for diagnosing leishmaniasis.
    • Understanding parasite localization in blood aids in comprehending disease pathogenesis.
    • Antimony's effect on parasite cultivability provides insights into treatment efficacy.