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ON THE DIALYSABILITY OF THE GROWTH-ACTIVATING PRINCIPLE CONTAINED IN EXTRACTS OF EMBRYONIC TISSUES

G P Wright1

  • 1Research Laboratories of the Barnard Free Skin and Cancer Hospital, and the Department of Surgery of Washington University School of Medicine, St. Louis.

The Journal of Experimental Medicine
|October 30, 2009
PubMed

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media