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STUDIES IN EXPERIMENTAL EXTRACORPOREAL THROMBOSIS : II. THROMBUS FORMATION IN NORMAL BLOOD IN THE EXTRACORPOREAL
1Division of Medicine, Mayo Clinic, and the Mayo Foundation, Rochester, Minnesota.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Extracorporeal circulation in rabbits quickly stops due to thrombus formation, primarily white thrombi obstructing cannulas. This study investigates the mechanisms and locations of thrombus development in normal animal models.
Area of Science:
- Hematology
- Vascular Biology
- Experimental Medicine
Background:
- Thrombus formation is a critical factor in circulatory system function and dysfunction.
- Understanding thrombus development in vivo is essential for developing therapeutic strategies.
- Extracorporeal circulation models provide a platform to study acute thrombotic events.
Purpose of the Study:
- To investigate the mechanisms and characteristics of thrombus formation in normal rabbits undergoing extracorporeal circulation.
- To identify the primary sites and types of thrombi that lead to circulatory cessation.
- To correlate blood flow dynamics with thrombus morphology and location.
Main Methods:
- Establishment of an experimental extracorporeal circulation model in normal rabbits.
- Observation of circulation cessation times and identification of obstructive masses.
- Analysis of thrombus composition (white thrombi, fibrin) and location (cannula, collodion tubes, jugular vein).
- Correlation of blood stream velocity with thrombus formation patterns.
Main Results:
- Circulation typically ceased within 6-10 minutes due to cannula obstruction by white thrombi and fibrin.
- Red mural thrombi were observed in collodion tubes, with a wide base on white thrombi.
- Rapid obstruction or slow blood flow could lead to clotting before significant platelet deposition or white thrombus formation.
- Mixed thrombi, originating from white thrombi or injured intima, were found in the jugular vein, extending downstream.
Conclusions:
- Thrombus formation, particularly white thrombi obstructing cannulas, is the primary cause of circulation cessation in this extracorporeal model.
- Blood flow dynamics significantly influence the type and location of thrombus formation.
- The study elucidates the sequence of events in acute thrombus development within an artificial circulatory loop.
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