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Updated: Jun 19, 2026
12:23
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
ON PRECIPITABLE SUBSTANCES DERIVED FROM BACILLUS TYPHOSUS AND BACILLUS PARATYPHOSUS B
1Laboratories of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Researchers failed to isolate ether-soluble substances from B. typhosus. They identified two protein substances and one non-protein substance with distinct properties, including resistance to digestion.
Area of Science:
- Microbiology
- Immunology
- Biochemistry
Background:
- Previous studies suggested the presence of ether-soluble specific substances in B. typhosus.
- These substances were thought to be serologically active and important for bacterial identification.
Purpose of the Study:
- To investigate the presence and nature of specific substances in B. typhosus.
- To characterize the serological and chemical properties of isolated substances.
- To compare substances from B. typhosus with those from related bacteria.
Main Methods:
- Extraction of substances from B. typhosus.
- Tryptic digestion and alkaline hypochlorite treatment to assess substance resistance.
- Alcohol extraction of protein substances.
- Preparation of specific precipitable substances from B. paratyphosus B, B. enteritidis, and Proteus strains.
- Serological analysis of the prepared substances.
Main Results:
- Attempts to isolate ether-soluble specific substances from B. typhosus were unsuccessful.
- Two serologically active protein substances and one non-protein substance were isolated.
- The protein substances were susceptible to tryptic digestion and alkaline hypochlorite, while the non-protein substance was resistant.
- One protein substance was extractable with 75% alcohol.
- Specific precipitable substances containing carbohydrates, similar to the B. typhosus non-protein substance, were prepared from B. paratyphosus B, B. enteritidis, and Proteus strains.
Conclusions:
- Ether-soluble specific substances could not be confirmed in B. typhosus.
- B. typhosus contains distinct protein and non-protein substances with differential resistance to chemical treatments.
- Carbohydrate-rich substances with similar serological reactivity to the B. typhosus non-protein substance can be isolated from other enteric bacteria.
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