THE USE OF HISTAMINE AS A STANDARD TEST FOR DIMINISHED RESISTANCE IN SUPRARENALECTOMIZED RATS

Area of Science:

• Pharmacology
• Physiology
• Endocrinology

Background:

• Suprarenalectomy (adrenalectomy) in rats leads to physiological changes affecting drug resistance.
• Previous drug testing for suprarenalectomized rats yielded unsatisfactory results.
• Histamine's properties suggest potential utility in this research context.

Purpose of the Study:

• To identify the most suitable drug for assessing the resistance of suprarenalectomized rats.
• To evaluate histamine as a potential agent for this purpose.

Main Methods:

• Testing various drugs on suprarenalectomized rat models.
• Establishing the Minimum Lethal Dose (M.L.D.) of histamine for both normal and suprarenalectomized rats.
• Comparing the M.L.D. values between the two groups.

Main Results:

• Histamine proved to be the most satisfactory drug among those tested.
• Histamine is readily available, stable, and induces characteristic symptoms.
• The M.L.D. of histamine was established within narrow limits for a specific rat strain.
• A significant difference (1:20 ratio) was observed in the M.L.D. between normal and suprarenalectomized rats.

Conclusions:

• Histamine is a highly effective and reliable agent for testing the resistance of suprarenalectomized rats.
• The pronounced difference in M.L.D. highlights histamine's utility in studying adrenal insufficiency models.