THE EFFECT OF OXIDATION OF FILTRATES OF A CHICKEN SARCOMA (CHICKEN TUMOR I-ROUS)

Area of Science:

• Oncology
• Virology
• Biochemistry

Background:

• Rous Foul Sarcoma I filtrates are used in experimental research.
• Filtrates exhibit rapid autoinactivation at physiological temperatures.
• Oxidative processes are suspected contributors to filtrate instability.

Purpose of the Study:

• To investigate the cause of rapid autoinactivation of Rous Foul Sarcoma I filtrates.
• To identify methods for preventing oxidative degradation of filtrates.
• To enhance the stability and infectiousness of viral extracts for experimental use.

Main Methods:

• Preparation and handling of candle filtrates from Rous Foul Sarcoma I.
• Incubation of filtrates at 37°C to observe autoinactivation.
• Application of cysteine hydrochloride (1:2000 dilution, pH 7.4) to prevent oxidation.
• Assessment of filtrate infectivity and stability under different conditions.

Main Results:

• Autoinactivation of Rous Foul Sarcoma I filtrates at 37°C is primarily due to oxidation.
• Cysteine hydrochloride effectively prevents oxidative inactivation when filtrates are diluted (1:2000) and buffered to pH 7.4.
• Preventing oxidative changes during preparation results in more active and consistent viral extracts.
• Preservation in an ice box with cysteine and vaseline allows for extended storage of infective filtrates.

Conclusions:

• Oxidation is a major factor in the instability of Rous Foul Sarcoma I filtrates.
• Cysteine hydrochloride is a potent antioxidant that stabilizes these viral preparations.
• Optimized preparation and storage methods using cysteine improve the reliability of experimental results with Rous Foul Sarcoma I.