Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pneumonia II: Pathophysiology

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:

Atypical Pneumonia

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...

Special Staining Techniques

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Studies on the Flexner group of dysentery bacilli; the serological and toxic properties of the somatic antigens.

The Journal of experimental medicine·2010

Same author

Studies on the Flexner group of dysentery bacilli; a quantitative study of the serological cross-reactions.

The Journal of experimental medicine·2010

Same author

Studies on the chemical nature of the substance inducing transformation of pneumococcal types; effect of desoxyribonuclease on the biological activity of the transforming substance.

The Journal of experimental medicine·2010

Same author

Studies on the chemical nature of the substance inducing transformation on pneumococcal types; an improved method for the isolation of the transforming substance and its application to Pneumococcus Types II, III, and VI.

The Journal of experimental medicine·2010

Same author

Inhibition of mumps virus multiplication by a polysaccharide.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010

Same author

Concerning the Bacteriemic Theory of Tuberculosis.

The Journal of medical research·2009

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

A STUDY OF PNEUMOCOCCUS AUTOLYSIS.

W F Goebel¹, O T Avery

¹Hospital of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine

|October 30, 2009

Summary

Pneumococcus autolysis involves proteolysis and lipolysis, releasing nitrogen and fatty acids. Bile salt solution rapidly dissolves pneumococci at low temperatures without enzymatic activity, suggesting it differs from autolysis.

Area of Science:

Microbiology
Biochemistry
Enzymology

Background:

Pneumococcus undergoes autolysis, a process involving cellular breakdown.
Autolysis is associated with the release of intracellular components and enzymatic activity.

Purpose of the Study:

To investigate the enzymatic processes, specifically proteolysis and lipolysis, during Pneumococcus autolysis.
To compare spontaneous autolysis with cell lysis induced by intracellular enzyme extracts.
To examine the effect of sodium desoxycholate on Pneumococcus lysis and its relationship to enzymatic activity.

Main Methods:

Analysis of nitrogenous compounds (amino and non-coagulable nitrogen) during autolysis.
Quantification of ether-soluble fatty acids released during lipolysis.

More Related Videos

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Published on: April 28, 2014

Related Experiment Videos

Last Updated: Jun 19, 2026

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Published on: April 28, 2014

Incubation of heat-killed pneumococci and lipoid emulsions with intracellular enzyme extracts.

Assessment of Pneumococcus lysis and enzymatic activity in the presence of sodium desoxycholate at low temperatures.

Main Results:

Autolysis of Pneumococcus results in increased amino and non-coagulable nitrogen (proteolysis) and liberation of ether-soluble fatty acids (lipolysis).
Intracellular enzyme extracts induced lysis of heat-killed pneumococci and lipoid emulsions, mirroring spontaneous autolysis.
Sodium desoxycholate inhibited pneumococcus protease but not lipase.
Rapid dissolution of pneumococci in sodium desoxycholate at 0°C occurred without significant lipolysis or proteolysis.

Conclusions:

Pneumococcus autolysis is characterized by coordinated proteolysis and lipolysis.
The mechanism of cell dissolution by sodium desoxycholate at low temperatures differs from spontaneous autolysis.
Enzyme action plays a crucial role in Pneumococcus autolysis and may relate to antigenic dissociation.