Related Experiment Videos
A procedure for cryopreservation of hamster oocytes yielding highly conserved oocytes suitable for sperm penetration
C Tobback1, S Hough, R H Foote
1Department of Animal Science, Cornell University Ithaca, New York 14853-4801.
Fertility and Sterility
|January 1, 1991
Abstract:
Hamsters were superovulated and their oocytes frozen in Dulbecco's phosphate-buffered saline containing 10% fetal calf serum and 1.5 molar 1,2-propanediol. In several experiments, 94% of the hamster oocytes survived freezing and were equivalent to fresh oocytes in sperm penetration. In routine use of the procedure described, also 94% of 1,340 frozen-thawed oocytes were satisfactory for sperm penetration of several species. Frozen oocytes eliminate problems and costs of maintaining hamster colonies with variable responses on individual days.