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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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FURTHER NOTES ON THE FILTRATION OF THE VIRUS OF VACCINIA
1Department of Bacteriology and Immunology, Harvard University Medical School, Boston.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Researchers developed a method to create an active vaccinia virus filtrate from lesions. This sterile filtrate retains significant infectivity and can be stored long-term, even frozen, without glycerol.
Area of Science:
- Virology
- Immunology
- Biotechnology
Background:
- Vaccinia virus is used in vaccination.
- Obtaining a stable and active viral preparation is crucial for vaccine development and research.
Purpose of the Study:
- To develop a method for obtaining an active and sterile filtrate from vaccinia lesions.
- To assess the infectivity and stability of the prepared filtrate.
Main Methods:
- Grinding vaccinia lesions with glass fragments.
- Emulsifying in hormone broth, followed by centrifugation.
- Filtering the supernatant through a Berkefeld V filter.
Main Results:
- A highly active filtrate was obtained from vaccinia lesions.
- The sterile filtrate showed approximately 1/16th the activity of the non-sterile vaccine emulsion.
- Centrifugation partially concentrated the virus in the lower layer.
- The virus remained viable for extended periods near freezing temperatures and indefinitely when frozen.
Conclusions:
- A reproducible method for preparing an active vaccinia virus filtrate was established.
- The filtrate maintains significant infectivity and stability under cold storage.
- Glycerol is not required for preserving the virus in the filtrate.
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