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Updated: Jun 19, 2026
07:06
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
THE EFFECT OF CATAPHORESIS ON POLIOMYELITIS VIRUS
P K Olitsky1, C P Rhoads, P H Long
1Laboratories of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Poliomyelitis virus carries a negative electrical charge, allowing it to be isolated from immune serum and detected in monkey nervous systems using cataphoresis.
Area of Science:
- Virology
- Neuroscience
- Immunology
Background:
- Poliomyelitis virus infectivity and behavior are influenced by its physical and chemical properties.
- Understanding virus charge is crucial for developing diagnostic and therapeutic strategies.
Purpose of the Study:
- To determine the electrical charge of the poliomyelitis virus.
- To explore the application of cataphoresis for virus recovery and detection.
Main Methods:
- Electrophoretic migration studies under varying hydrogen ion concentrations.
- Cataphoresis for isolating poliomyelitis virus from mixtures with immune serum.
- Cataphoresis for detecting virus presence in central nervous system tissue.
Main Results:
- Poliomyelitis virus exhibits electronegative charge, migrating to the anode in an electrical field.
- Cataphoresis successfully recovered infectious virus from non-infective virus-immune serum mixtures.
- Cataphoresis detected poliomyelitis virus in the central nervous system of recovered experimental subjects.
Conclusions:
- The electronegative charge of poliomyelitis virus is a key characteristic influencing its behavior.
- Cataphoresis is a viable method for isolating and detecting poliomyelitis virus.
- Virus presence can be confirmed in neural tissues even after recovery from experimental poliomyelitis.
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