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ON THE ISOLATION, CULTIVATION AND CLASSIFICATION OF THE SO-CALLED INTRACELLULAR "SYMBIONT" OR "RICKETTSIA" OF

¹Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

Researchers isolated and cultivated diphtheroidal bacilli, identified as Corynebacterium periplanetae nov. sp. variety americana, from the American cockroach (Periplaneta americana). These bacteria, found in bacteriocytes, are transmitted across generations.

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Area of Science:

Microbiology
Insect Pathology
Bacteriology

Background:

Bacteriocytes in Periplaneta americana harbor intracellular microorganisms.
These microorganisms are suspected to be diphtheroidal bacilli transmitted maternally.

Purpose of the Study:

To isolate and cultivate the intracellular microorganisms from Periplaneta americana.
To characterize the isolated bacteria and determine their taxonomic status.
To investigate the transmission and potential diversity of these bacteria.

Main Methods:

Development of a contamination-free tissue isolation method for Periplaneta.
Utilized a novel "spotting technique" for culturing bacteria from eggs and bacteriocytes.
Employed serial transfers, morphological, biochemical, and serological analyses for characterization.

Main Results:

Successfully isolated and cultivated bacteria from Periplaneta americana in approximately 14% of attempts.
Identified three morphologically distinct types of diphtheroidal bacilli, consistent with intracellular parasites.
Serological analysis revealed distinctions between the three types, supporting their classification as a single species, Corynebacterium periplanetae nov. sp. variety americana.

Conclusions:

The intracellular parasite of Periplaneta americana has been successfully isolated and cultivated.
The isolated bacterium is identified as Corynebacterium periplanetae nov. sp. variety americana.
Three distinct cultural types were observed, suggesting intraspecies variation within the host.