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Updated: Jun 19, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
STUDIES ON VITAL STAINING : I. SOME PROBLEMS IN COLORIMETRY. THE QUANTITATIVE ANALYSIS OF MIXTURES OF COLORED
1Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, N. Y.
Abstract:
1. A spectrophotometric method is discussed which permits quantitative analysis of colored substances present in mixtures. 2. Special attention is given to the analysis of mixtures of two dyes which are being used in a series of studies on vital staining. 3. It is shown that the method can be applied to quantitative analysis of mixtures of naturally occurring animal pigments or to mixtures of these with various other colored substances. 4. Certain limitations of the method and certain necessary precautions are discussed.
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