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Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
Published on: February 20, 2021
SURVIVAL AND MULTIPLICATION OF THE VIRUS OF POLIOMYELITIS IN VITRO
P H Long1, P K Olitsky, C P Rhoads
1Laboratories of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Researchers attempted to culture poliomyelitis virus in vitro. While minute bodies were observed, their viability was uncertain, yet subsequent transfers induced poliomyelitis in monkeys, suggesting in vitro multiplication.
Area of Science:
- Virology
- Microbiology
- Cell Culture
Background:
- Poliomyelitis virus was successfully inoculated into monkeys.
- Attempts were made to cultivate the virus from infected nervous tissues.
Purpose of the Study:
- To determine if poliomyelitis virus could be cultivated in vitro.
- To investigate the nature of observed minute bodies in cultures.
Main Methods:
- Bacteriological cultivation of nervous tissue fragments and filtrates from infected monkeys.
- Subplanting of observed minute bodies and inoculation of transfers into monkeys.
- Microscopic examination of tissue cells and leukocytes in cultures.
Main Results:
- Thirty-six out of 315 tubes showed minute bodies resembling globoid bodies.
- Subplants from these cultures, after multiple transfers, induced experimental poliomyelitis in monkeys.
- Pathogenicity was selective, correlating with the presence of minute bodies in later transfers.
Conclusions:
- The poliomyelitis virus appears to multiply in vitro within a modified living tissue-cell culture medium.
- The observed minute bodies may be associated with the virus, potentially through adsorption.
- Further research is needed to confirm the nature of these bodies and their role in virus propagation.
