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Published on: February 3, 2015
EXPERIMENTS ON THE PURIFICATION AND CONCENTRATION OF THE VIRUS OF POLIOMYELITIS
1Department of Bacteriology and Immunology, New York University and Bellevue Hospital Medical College, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Poliomyelitis virus can be purified and concentrated using alumina gel C adsorption and elution. This reversible process yields a purified virus capable of causing infection, facilitating chemical and immunological studies.
Area of Science:
- Virology
- Biochemistry
- Immunology
Background:
- Enzyme isolation techniques were adapted for poliomyelitis virus.
- Previous work showed alumina gel C adsorbs and inactivates the virus at acidic pH.
Purpose of the Study:
- To confirm and expand upon Rhoads' findings regarding poliomyelitis virus and alumina gel C.
- To develop methods for purifying and concentrating the poliomyelitis virus for further study.
Main Methods:
- Adsorption of poliomyelitis virus to alumina gel C at acidic pH.
- Reversible elution of the virus using alkaline conditions (M/15 Na(2)HPO(4)).
- Purification through repeated adsorption-elution cycles and vacuum distillation.
Main Results:
- Adsorption and inactivation of the virus were confirmed as reversible.
- A significant purification of the virus was achieved, reducing non-virus substances.
- A concentrated virus solution with a minimal effective dose of 0.0003 cc. was obtained, showing negative protein reactions.
Conclusions:
- The described methods allow for the preparation of purified and concentrated poliomyelitis virus.
- This purified virus is suitable for chemical nature studies.
- Alumina gel C provides a method for anchoring the virus for immunological studies.
