Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Blood Typing

Blood Typing

Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Production of Formed Elements

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A national study of Chinese youths' attitudes towards students with intellectual disabilities.

Journal of intellectual disability research : JIDR·2011

Same author

Propagation of Theiler's GD-VII mouse virus in tissue culture.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010

Same author

Propagation of rabies virus in tissue culture.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010

Same author

THE RACES THAT CONSTITUTE THE GROUP OF COMMON FIBROBLASTS : I. THE EFFECT OF BLOOD PLASMA.

The Journal of experimental medicine·2009

Same author

THE RACES THAT CONSTITUTE THE GROUP OF COMMON FIBROBLASTS : III. DIFFERENCES DETERMINED BY ORIGIN OF EXPLANT AND AGE OF DONOR.

The Journal of experimental medicine·2009

Same author

STUDIES ON ORGANOGENESIS : I. THE ABILITY OF ISOLATED BLOOD CELLS TO FORM ORGANIZED VESSELS IN VITRO.

The Journal of experimental medicine·2009

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

THE RACES THAT CONSTITUTE THE GROUP OF COMMON FIBROBLASTS : II. THE EFFECT OF BLOOD SERUM.

¹Laboratories of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

Fibroblast growth in serum varies by cell strain and serum type. Initial serum exposure can be injurious, but cells adapt, showing strain-dependent proliferation and heteromorphic colony formation.

More Related Videos

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Published on: July 29, 2019

Related Experiment Videos

Last Updated: Jun 19, 2026

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Published on: July 29, 2019

Area of Science:

Cell Biology
Tissue Culture

Background:

Fibroblasts are connective tissue cells with proliferative capacity.
Serum is a common medium component for cell culture, influencing cell behavior.

Purpose of the Study:

To investigate fibroblast proliferation and colony morphology in various serum-based media.
To understand the influence of serum composition and cell strain on fibroblast growth dynamics.

Main Methods:

Culturing fibroblasts in different serum preparations (plain, heparinized, plasma).
Observing fibroblast proliferation rates and colony morphology over time.
Comparing growth in serum versus media with embryonic tissue juice.

Main Results:

Fibroblast multiplication in serum is sustained but slow, highly dependent on cell strain's inherent growth energy.
Initial serum application causes injury, which diminishes as cells adapt and proliferate.
Fibroblast colonies in serum are heteromorphic, unlike the isomorphic colonies formed in media with growth-activating substances.

Conclusions:

Cell strain characteristics are critical determinants of fibroblast proliferation rates in serum.
Serum's initial detrimental effect on fibroblasts is transient, with subsequent adaptation and improved growth.
Serum promotes cellular heterogeneity within fibroblast colonies, contrasting with the uniformity seen in growth-factor-enriched media.