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FAILURE TO NEUTRALIZE THE POLIOMYELITIS VIRUS WITH SERA OF ADULT MACACUS RHESUS AND OF YOUNG FEMALE RHESUS TREATED
N P Hudson1, E H Lennette, E Q King
1Department of Hygiene and Bacteriology and the Department of Medicine of the Division of Biological Sciences, The University of Chicago, Chicago.
Abstract:
1. Twelve specimens of serum from nine adult male monkeys failed to neutralize the virus of poliomyelitis. 2. Ten samples of serum obtained from three adult female monkeys at various phases of the menstrual cycle likewise proved incapable of neutralizing the virus. An eleventh serum, drawn from a fourth female 32 days postpartum, gave irregular results. It neutralized once and failed to do so on second test. This is the only suggestion in our experiments that a physiological factor may play a part in poliomyelitis immunity. 3. Fourteen sera from ten immature monkeys caused to menstruate by treatment with anterior pituitary extract were devoid of virucidal property. This treatment failed also to induce a systemic resistance to intracerebral injections of virus in the nine monkeys of the same group available for test. 4. We were unable to demonstrate in our monkeys a correlation between virucidal capacity of the serum and maturity or physiological variations as exemplified by menstruation.
Insights
This study investigated poliomyelitis virus neutralization in monkey serum, finding no correlation with maturity or menstruation. Physiological factors in poliomyelitis immunity were not definitively demonstrated in the tested monkey models.
Area of Science:
- Virology
- Immunology
- Primatology
Background:
- Poliomyelitis remains a significant public health concern.
- Understanding viral neutralization is crucial for developing effective vaccines and treatments.
- Investigating potential natural immunity factors in animal models can provide insights into human susceptibility.
Purpose of the Study:
- To determine if monkey serum can neutralize the poliomyelitis virus.
- To explore the potential influence of physiological factors, such as maturity and the menstrual cycle, on poliomyelitis immunity in monkeys.
- To assess the impact of induced menstruation on viral resistance.
Main Methods:
- Sera from adult male and female monkeys, including those at various menstrual cycle phases and postpartum, were tested for poliomyelitis virus neutralizing capacity.
- Immature monkeys were treated with anterior pituitary extract to induce menstruation, and their sera were subsequently tested.
- Systemic resistance to intracerebral virus injection was evaluated in treated immature monkeys.
Main Results:
- Sera from adult male and most adult female monkeys did not neutralize the poliomyelitis virus.
- One postpartum female monkey's serum showed inconsistent neutralization results.
- Sera from immature monkeys, even after induced menstruation, lacked virucidal properties, and induced menstruation did not confer systemic resistance.
Conclusions:
- No correlation was found between serum's virucidal capacity and maturity or physiological variations like menstruation in the studied monkeys.
- The study did not demonstrate a significant role for these physiological factors in poliomyelitis immunity in this model.
- Further research is needed to identify factors contributing to poliomyelitis immunity.
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