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Updated: Jun 19, 2026

Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : I. PREPARATION OF ELEMENTARY BODIES OF VACCINIA
1Hospital of The Rockefeller Institute for Medical Research.
Abstract:
By means of differential centrifugation in the horizontal and angle centrifuges it is possible to obtain appreciable quantities of relatively pure elementary bodies of vaccinia. Such preparations of elementary bodies exhibit an extremely high titer of vaccine virus. The elementary bodies are specifically agglutinated by serum from rabbits immunized by means of injections of bacteria-free vaccine virus. Virus-free filtrates of dermal vaccine virus and of bacteria-free testicular vaccine virus contain a soluble substance or substances that is precipitated by antivaccinal sera.
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