Related Experiment Video
Updated: Jun 19, 2026
10:03
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
Published on: November 5, 2019
STUDIES ON MENINGOCOCCUS INFECTION : IX. STANDARDIZATION AND CONCENTRATION OF ANTIMENINGOCOCCUS HORSE SERUM (TYPE I).
1Laboratories of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Type I antimeningococcal horse sera were standardized using precipitin content. A novel purification method concentrated antibodies tenfold, improving diagnostic accuracy for meningococcal disease.
Area of Science:
- Immunology
- Microbiology
- Serology
Background:
- Standardization of antisera is crucial for accurate diagnostic tests.
- Type I antimeningococcal horse sera are used in diagnostics.
- Previous methods for antibody purification were less efficient.
Purpose of the Study:
- To standardize Type I antimeningococcal horse sera.
- To develop an efficient method for purifying antimeningococcal antibodies.
Main Methods:
- Quantitative determination of type-specific precipitin content.
- Antibody purification using dialysis and carbon dioxide precipitation.
Main Results:
- The purification method achieved a tenfold increase in antibody concentration relative to nitrogen content.
- Standardization of Type I antimeningococcal horse sera was successfully performed.
Conclusions:
- The developed purification technique is effective for concentrating antimeningococcal antibodies.
- Standardized sera enhance the reliability of diagnostic assays for meningococcal infections.
Related Concept Videos
01:26Bacterial Meningitis II: Pathophysiology
Bacterial meningitis typically begins when pathogens such as Neisseria meningitidis and Streptococcus pneumoniae colonize the nasopharynx and invade the bloodstream. This process is facilitated by bacterial virulence factors, such as polysaccharide capsules, which resist phagocytosis and complement-mediated killing. Less commonly, bacteria reach the central nervous system via contiguous spread from infections like otitis media or sinusitis, through congenital or acquired dural defects, or...
01:22Bacterial Meningitis I: Introduction
Bacterial meningitis is a severe, life-threatening inflammation of the meninges, particularly the pia mater and arachnoid mater, affecting the subarachnoid space, ventricles, and cerebrospinal fluid (CSF). If untreated, it can lead to significant neurological complications or death.Causative AgentsCommon pathogens vary with age and immune status. In adults, major organisms include Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae. Streptococcus agalactiae (group B...
01:18Viral Meningitis
Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...
01:24Bacterial Meningitis
Bacterial meningitis is a severe infectious disease involving inflammation of the meninges, the protective membranes surrounding the brain and spinal cord. It occurs when pathogenic bacteria cross the blood–brain barrier and enter the cerebrospinal fluid. Common causative organisms include Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae type b, Listeria monocytogenes, and Escherichia coli K1. The exact route of entry varies by pathogen and host condition.Routes of Entry...