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Updated: Jun 19, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

AN AIR-DRIVEN ULTRACENTRIFUGE FOR MOLECULAR SEDIMENTATION.

J Biscoe¹, E G Pickels, R W Wyckoff

¹Laboratories of The Rockefeller Institute for Medical Research, and the Laboratories of the International Health Division of the Rockefeller Foundation, New York.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

This study details the construction of an air-driven ultracentrifuge for molecular sedimentation. The new design features rotors with a 6.50 cm radius and advanced cameras for improved analysis.

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Area of Science:

Biophysical chemistry
Analytical chemistry
Biotechnology

Background:

Ultracentrifugation is a key technique for analyzing macromolecular properties.
Existing oil-driven ultracentrifuges, like Svedberg's, are effective but can be complex.
Development of alternative ultracentrifuge designs is crucial for broader accessibility and application.

Purpose of the Study:

To describe the construction of a novel air-driven ultracentrifuge.
To present an alternative to traditional oil-driven ultracentrifugation methods.
To highlight the instrument's design features for molecular sedimentation analysis.

Main Methods:

Detailed construction blueprints and methodologies for an air-driven ultracentrifuge.
Incorporation of rotors with a 6.50 cm radius of rotation.
Utilization of cameras with a great depth of focus for enhanced imaging.

Main Results:

Successful construction of a functional air-driven ultracentrifuge.
Demonstration of the instrument's capability for molecular sedimentation.
Achieved performance comparable to standard oil-driven ultracentrifuges.

Conclusions:

The air-driven ultracentrifuge offers a viable alternative for molecular sedimentation studies.
The design incorporates features for improved analytical performance.
This instrument can advance research in macromolecular characterization.