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Updated: Jun 19, 2026

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
Published on: February 7, 2019
CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES :
G Shwartzman1, S Morell, H Sobotka
1Laboratories of The Mount Sinai Hospital, New York.
Abstract:
A quantitative biological assay of the products obtained from the dialysis of B. typhosus, meningococcus, and B. coli culture filtrates has been undertaken. It was found that the active principles of the phenomenon of local skin reactivity to bacterial filtrates were retained by cellophane membranes. An appreciable purification was thus effected, amounting, on the average, to about a threefold increase in reacting potency per milligram of dry weight and of nitrogen. Attempts to purify bacterial filtrates by the fractional precipitation of their concentrates with dilute alkali, acid, and alcohol were unsuccessful.
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