Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Allergic Reactions: Anaphylaxis

Allergic Reactions: Anaphylaxis

Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...

Allergic Reactions

Allergic Reactions

Rheumatic Heart Disease I: Introduction

Rheumatic Heart Disease I: Introduction

Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...

Hypersensitivities

Hypersensitivities

Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...

Allergic Drug Reactions

Allergic Drug Reactions

Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...

Drug Toxicity: Allergic Reactions

Drug Toxicity: Allergic Reactions

Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial exposure to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Delirium in Association with Myocardial Insufficiency.

Transactions of the American Clinical and Climatological Association·2011

Same author

The Renal Manifestations of Obstruction of the Lower Urinary Tract.

Transactions of the American Climatological and Clinical Association. American Climatological and Clinical Association·2011

Same author

Private clinics in medical schools.

Journal. Association of American Medical Colleges·2010

Same author

A Reducing Substance Found in Chromophilic Adenomas and in the Normal Anterior Pituitary.

The American journal of pathology·2009

Same author

Clinical Experience with Powdered Leaves of Digitalis.

Proceedings of the Royal Society of Medicine·2009

Same author

THE ACTION OF THE SYMPATHETIC UPON THE EXCITATORY PROCESS IN THE MAMMALIAN HEART.

The Journal of experimental medicine·2009

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Measuring Local Anaphylaxis in Mice

Measuring Local Anaphylaxis in Mice

Published on: October 14, 2014

ANAPHYLAXIS IN THE ISOLATED HEART.

H B Wilcox¹, E C Andrus

¹Cardiographic Laboratory, Department of Medicine, Johns Hopkins University and Hospital, Baltimore.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

Guinea pig hearts sensitized to horse serum showed characteristic anaphylactic reactions, including altered heart rate, contraction, and reduced coronary blood flow. Histamine effects and atropine

More Related Videos

Isolation of Functional Cardiac Immune Cells

Isolation of Functional Cardiac Immune Cells

Published on: December 5, 2011

Isolation and Identification of Extravascular Immune Cells of the Heart

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

Related Experiment Videos

Last Updated: Jun 19, 2026

Measuring Local Anaphylaxis in Mice

Measuring Local Anaphylaxis in Mice

Published on: October 14, 2014

Isolation of Functional Cardiac Immune Cells

Isolation of Functional Cardiac Immune Cells

Published on: December 5, 2011

Isolation and Identification of Extravascular Immune Cells of the Heart

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

Area of Science:

Cardiovascular Physiology
Immunology
Pharmacology

Background:

Anaphylactic shock in intact animals causes electrocardiographic abnormalities.
Isolated organ preparations are valuable for studying specific physiological responses.

Purpose of the Study:

To characterize the anaphylactic reaction in isolated guinea pig hearts.
To compare anaphylaxis with histamine effects on the isolated heart.
To investigate the influence of atropine on these reactions.

Main Methods:

Utilized isolated, perfused guinea pig hearts sensitized to horse serum.
Exposed hearts to antigen and recorded cardiac rate, contraction amplitude, and coronary flow.
Administered histamine and atropine to assess their effects.

Main Results:

Sensitized isolated hearts exhibited transient cardiac acceleration and altered contraction amplitude upon antigen exposure.
Electrocardiographic abnormalities were recorded directly from the isolated heart.
A significant reduction in coronary vessel flow was observed during anaphylaxis.

Conclusions:

The isolated guinea pig heart preparation effectively models anaphylactic cardiovascular responses.
Anaphylaxis and histamine induce distinct, yet comparable, effects on the isolated heart.
Quantitative differences in atropine's effects suggest specific receptor interactions.