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L E Baker1, A H Ebeling

  • 1Laboratories of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine
|October 30, 2009
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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media