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Updated: Jun 19, 2026
Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
J E Smadel1, T M Rivers, E G Pickels
1Hospital of The Rockefeller Institute for Medical Research, and the Laboratories of the International Health Division of The Rockefeller Foundation, New York.
Abstract:
A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by means of a calculated value for the weight of a single dehydrated elementary body. Values for the ratio of infective units of vaccine virus to elementary bodies varied between 1 : 2.4 and 1 : 9.2 in seven consecutive experiments; the average was 1:4.2. These ratios indicate a high degree of purity of the preparation. Moreover, they indicate that a relatively high percentage of the elementary bodies in the preparations was infective.
