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BLOOD PLASMA PROTEIN PRODUCTION AND UTILIZATION : THE INFLUENCE OF AMINO ACIDS AND OF STERILE ABSCESSES.
S C Madden1, C A Finch, W G Swalbach
1Department of Pathology, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Dogs with depleted plasma proteins can maintain normal health, revealing dietary needs for protein synthesis. Specific amino acids like cystine are crucial for efficient plasma protein regeneration, highlighting the dynamic nature of body proteins.
Area of Science:
- Biochemistry
- Physiology
- Nutrition
Background:
- Plasma protein levels are critical for maintaining physiological balance.
- Understanding protein metabolism is key to addressing conditions like hypoproteinemia.
Purpose of the Study:
- To investigate the factors influencing plasma protein production in dogs.
- To identify essential dietary components for plasma protein synthesis and regeneration.
Main Methods:
- Inducing experimental hypoproteinemia in dogs via plasmapheresis.
- Administering various protein sources and amino acid supplements in controlled diets.
- Monitoring plasma protein levels and nitrogen balance.
Main Results:
- Casein supported significant plasma protein production (33% of protein fed).
- Zein alone had no effect, but supplementation with cystine, tryptophan, lysine, and glycine doubled protein production.
- Liver protein combined with cystine, leucine, glutamic acid, and glycine was as effective as oral plasma protein (40% utilization).
- Cystine, leucine, and glutamic acid were identified as primary importance for plasma protein synthesis.
Conclusions:
- Plasma protein formation depends on both dietary intake and body reserves.
- Body proteins, including plasma proteins, exist in a dynamic equilibrium, readily shifting between depots.
- Specific amino acid profiles are essential for efficient plasma protein regeneration.
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