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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
SOME CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA
J E Smadel1, G I Lavin, R J Dubos
1Hospital and the Laboratories of The Rockefeller Institute for Medical Research.
Abstract:
Treatment of elementary bodies of vaccinia with dilute solutions of sodium hydroxide resulted in the extraction of certain soluble materials accounting for half of the dry weight of the virus. Elementary bodies contained about 0.4 per cent inorganic phosphorus, practically all of which occurred in the form of a nucleoprotein containing thymus nucleic acid. In addition, a substance was recovered that reacted with S antibodies. From past experience one is led to believe that S antigen, as ordinarily encountered, is a protein which is not in combination with nucleic acid.
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