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ANTIBODY FORMATION IN A TUBERCULOUS LESION AT THE SITE OF INOCULATION
1Department of Pathology, Cornell University Medical College, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Antibody formation occurs locally at the injection site of heat-killed tubercle bacilli in oil, appearing before in blood serum. This local antibody production is supported by evidence of higher concentrations at the lesion site.
Area of Science:
- Immunology
- Microbiology
Background:
- Antibody presence in lesions following antigen injection is a key indicator of immune response.
- Understanding the kinetics and location of antibody production is crucial for vaccine development and understanding infectious diseases.
Purpose of the Study:
- To investigate the site and kinetics of complement-fixing antibody production after injection of heat-killed tubercle bacilli in oil.
- To determine if local antibody accumulation is due to serum antibody fixation or local synthesis.
Main Methods:
- Animals were injected with heat-killed tubercle bacilli in oil.
- Complement fixation tests were performed on tissue extracts from the injection site and blood serum.
- Lesion extracts from control injections (paraffin oil, old tuberculin, cod liver oil) were also analyzed.
Main Results:
- Complement-fixing antibody appeared earlier and in higher concentrations at the injection site lesion compared to blood serum for approximately 3 weeks.
- Antibody levels at the injection site correlated with the quantity of bacilli injected, up to a maximum.
- Control lesions showed no or very low antibody levels, even with high serum titers, refuting systemic antibody fixation as the cause of local accumulation.
Conclusions:
- Antibody is synthesized locally in the skin at the site of tubercle bacilli injection.
- The findings suggest active, localized immune response rather than passive accumulation of circulating antibodies.
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