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THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
G L Hobby1, M H Dawson, K Meyer
1Departments of Medicine and Ophthalmology, College of Physicians and Surgeons, Columbia University, the Edward Daniels Faulkner Arthritis Clinic, and the Institute of Ophthalmology, Presbyterian Hospital, New York.
This study compared spreading factor and hyaluronidase, finding they share similarities but are not identical. Hyaluronidase may contribute to spreading, but the reaction is more complex than a simple chemical process.
Area of Science:
- Biochemistry
- Enzymology
- Dermatology
Background:
- Spreading factor and hyaluronidase are enzymes involved in biological processes.
- Hyaluronic acid is a key component in connective tissues, influencing tissue hydration and structure.
Purpose of the Study:
- To comparatively analyze the similarities and dissimilarities between spreading factor and hyaluronidase.
- To elucidate the mechanism of the spreading reaction and the role of hyaluronidase.
Main Methods:
- Comparative analysis of enzyme preparations from various sources.
- Thermal inactivation studies at 65°C and 100°C.
- Investigation using antisera to specifically inhibit enzyme activity.
Main Results:
- Preparations with hyaluronidase consistently exhibited spreading activity.
- Both spreading factor and hyaluronidase showed comparable inactivation upon heating.
- However, not all spreading factors contained hyaluronidase, and antisera against hyaluronidase did not inhibit spreading factor activity.
Conclusions:
- Hyaluronidase activity may contribute to the spreading reaction, supported by the presence of hyaluronic acid in skin.
- The spreading phenomenon is complex and not solely attributable to hyaluronidase activity.
- Further research is needed to fully understand the multifaceted nature of the spreading reaction.
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