Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determinants of Bacterial Pathogenicity and Virulence

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...

Hydrolysis

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Basic considerations with respect to antituberculous chemotherapy].

Revista mexicana de tuberculosis y enfermedades del aparato respiratorio·2014

Same author

Observations on the mode of action of oleandomycin.

Antibiotics & chemotherapy (Northfield, Ill.)·2014

Same author

The effect of sulfonamides on the action of penicillin.

Journal of bacteriology·2010

Same author

Factors influencing the action of streptomycin in vitro.

Journal of bacteriology·2010

Same author

Excretion of penicillin in human milk following parturition.

American journal of obstetrics and gynecology·2010

Same author

Observations on the action of streptomycin in vitro.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Published on: July 28, 2022

THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE.

G L Hobby¹, M H Dawson, K Meyer

¹Departments of Medicine and Ophthalmology, College of Physicians and Surgeons, Columbia University, the Edward Daniels Faulkner Arthritis Clinic, and the Institute of Ophthalmology, Presbyterian Hospital, New York.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

This study compared spreading factor and hyaluronidase, finding they share similarities but are not identical. Hyaluronidase may contribute to spreading, but the reaction is more complex than a simple chemical process.

More Related Videos

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Related Experiment Videos

Last Updated: Jun 19, 2026

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Published on: July 28, 2022

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Area of Science:

Biochemistry
Enzymology
Dermatology

Background:

Spreading factor and hyaluronidase are enzymes involved in biological processes.
Hyaluronic acid is a key component in connective tissues, influencing tissue hydration and structure.

Purpose of the Study:

To comparatively analyze the similarities and dissimilarities between spreading factor and hyaluronidase.
To elucidate the mechanism of the spreading reaction and the role of hyaluronidase.

Main Methods:

Comparative analysis of enzyme preparations from various sources.
Thermal inactivation studies at 65°C and 100°C.
Investigation using antisera to specifically inhibit enzyme activity.

Main Results:

Preparations with hyaluronidase consistently exhibited spreading activity.
Both spreading factor and hyaluronidase showed comparable inactivation upon heating.
However, not all spreading factors contained hyaluronidase, and antisera against hyaluronidase did not inhibit spreading factor activity.

Conclusions:

Hyaluronidase activity may contribute to the spreading reaction, supported by the presence of hyaluronic acid in skin.
The spreading phenomenon is complex and not solely attributable to hyaluronidase activity.
Further research is needed to fully understand the multifaceted nature of the spreading reaction.