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CASEIN DIGESTS PARENTERALLY UTILIZED TO FORM BLOOD PLASMA PROTEIN
S C Madden1, L J Zeldis, A D Hengerer
1Department of Pathology, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Dogs with depleted plasma proteins can effectively produce new plasma proteins using casein digests. Intravenous casein digests are as effective as oral liver for protein synthesis in these dogs.
Area of Science:
- Biochemistry
- Physiology
- Nutrition
Background:
- Plasmapheresis in dogs leads to hypoproteinemia and reduced resistance to infection.
- A steady state of hypoproteinemia allows for uniform plasma protein production on a limited protein diet.
Purpose of the Study:
- To investigate the efficacy of casein digests in stimulating plasma protein production in dogs with depleted plasma proteins.
- To compare the effectiveness of intravenous and subcutaneous administration of casein digests.
- To evaluate the role of specific amino acids (tryptophan, cysteine) in protein synthesis.
Main Methods:
- Dogs underwent plasmapheresis to induce hypoproteinemia.
- Casein digests and whole liver were administered intravenously or orally.
- Plasma protein production and amino acid retention were measured.
Main Results:
- Intravenous casein digests effectively promoted new plasma protein production in depleted dogs.
- Casein digest L administered intravenously was equivalent to oral whole liver for protein synthesis.
- Protein digest X required tryptophan and cysteine supplementation for efficacy.
- Cysteine sulfur was highly retained (>95%) in dogs.
Conclusions:
- Casein digests are a viable method for stimulating plasma protein synthesis in hypoproteinemic dogs.
- Intravenous administration of casein digests can be as effective as oral intake.
- Specific amino acid supplementation is crucial for certain protein digests to be effective.
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