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Clonogenic Assay: Adherent Cells
Published on: March 13, 2011
THE ROENTGEN RADIATION OF PAPILLOMA VIRUS (SHOPE) : II. THE EFFECT OF X-RAYS UPON PAPILLOMA VIRUS IN VITRO
J T Syverton1, G P Berry, S L Warren
1Departments of Bacteriology and Medicine (Radiology), University of Rochester, School of Medicine and Dentistry, Rochester, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Shope papilloma virus requires extremely high Roentgen irradiation doses for inactivation, far exceeding those for other agents or papilloma eradication. These large doses reduce virus titer and tumor development in rabbits.
Area of Science:
- Virology
- Radiation Biology
- Oncology
Background:
- Shope papilloma virus causes skin tumors in rabbits.
- Infectious agents vary in their susceptibility to radiation inactivation.
Purpose of the Study:
- To determine the Roentgen irradiation dose required for in vitro inactivation of Shope papilloma virus.
- To compare the radiosensitivity of Shope papilloma virus with other infectious agents and its in vivo effect on papillomas.
Main Methods:
- In vitro inactivation of cell-free Shope papilloma virus suspensions using varying doses of Roentgen irradiation.
- In vivo assessment of Roentgen irradiation's effect on papilloma development in domestic rabbits.
Main Results:
- Shope papilloma virus requires millions of r units for in vitro inactivation, significantly higher than other agents.
- This dose is thousands of times greater than that needed to eradicate papillomas in vivo.
- Large Roentgen doses reduced virus titer, increased latency period, and decreased papilloma size.
Conclusions:
- Shope papilloma virus exhibits remarkable radioresistance in vitro.
- High-dose Roentgen irradiation can inhibit papilloma virus activity and tumor formation.
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