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Updated: Jun 19, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

THE MANUFACTURE OF ANTIBODIES IN VITRO.

L Pauling¹, D H Campbell

¹Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena, California.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

Researchers developed a novel method to create specific antibodies, or antisera, from proteins. This technique successfully generated antisera against dyes and bacterial polysaccharides, demonstrating a new approach in antibody production.

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Published on: February 6, 2018

Area of Science:

Immunology
Biochemistry
Protein Chemistry

Background:

Traditional antibody production can be complex and time-consuming.
Developing specific antisera is crucial for diagnostic and research applications.

Purpose of the Study:

To investigate a novel method for generating specific antisera using protein denaturation and renaturation.
To demonstrate the efficacy of this method with various antigens, including dyes and polysaccharides.

Main Methods:

Treatment of bovine gamma-globulin with alkali in the presence of methyl blue dye, followed by neutralization.
Heating solutions of gamma-globulin and antigen to 57°C for several days.
Testing the resulting antisera for specific binding and precipitation reactions.

Main Results:

Successfully produced a protein solution with antiserum properties against methyl blue dye.
Generated homologous antisera against an azodye and pneumococcus polysaccharide Type III.
The antipneumococcus sera specifically precipitated Type III polysaccharide and agglutinated Type III pneumococci, showing no cross-reactivity with Types I and VIII (precipitation) or Types I and II (agglutination).

Conclusions:

The alkali-induced denaturation-renaturation and heat-induced methods are effective for preparing specific antisera.
This approach offers a viable alternative for producing targeted antibodies against various antigens.