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Related Experiment Video

Updated: Jun 19, 2026

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

EPIDEMIC KERATOCONJUNCTIVITIS : I. ISOLATION AND IDENTIFICATION OF A FILTERABLE VIRUS.

M Sanders1, R C Alexander

  • 1Departments of Ophthalmology and Bacteriology, College of Physicians and Surgeons, Columbia University, and the Institute of Ophthalmology, Presbyterian Hospital, New York.

The Journal of Experimental Medicine
|October 30, 2009
PubMed
Summary

A novel virus causing epidemic keratoconjunctivitis was isolated and characterized. This virus is neurotropic and can be neutralized by convalescent patient serum, confirming its role in the disease.

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Area of Science:

  • Ophthalmology
  • Virology
  • Infectious Diseases

Background:

  • Epidemic keratoconjunctivitis (EK) is a contagious eye infection.
  • The causative agent of EK was previously unidentified.

Purpose of the Study:

  • To isolate and characterize the virus responsible for epidemic keratoconjunctivitis.
  • To confirm the etiological role of the isolated virus in human disease.

Main Methods:

  • Virus isolation from patient conjunctival scrapings and subsequent propagation in tissue culture and mouse models.
  • Pathogenicity studies in mice and rabbits via various inoculation routes.
  • Filtration studies using Seitz, Berkefeld, and collodion membranes.
  • Neutralization assays with patient and control sera, as well as hyperimmune animal sera.

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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

Related Experiment Videos

Last Updated: Jun 19, 2026

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
07:35

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

  • Experimental inoculation of a human volunteer.

    • Main Results:

    • A virus was successfully isolated and adapted to mice, exhibiting neurotropic properties and causing fatal disease.
    • The virus demonstrated specific pathogenicity in young mice and rabbits.
    • Filtration studies indicated the virus particle size.
    • Serum neutralization tests confirmed the etiological link between the isolated virus and EK.
    • A human volunteer developed characteristic EK symptoms upon inoculation with the mouse-adapted virus, with subsequent antibody development.

    Conclusions:

    • The isolated virus is the causative agent of epidemic keratoconjunctivitis.
    • The virus possesses neurotropic characteristics and can be identified through serological neutralization.
    • Successful experimental infection in a human volunteer validates the findings and provides a model for further study.