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Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria
J Barbe1, A R Fernandez de Henestrosa, S Calero
1Department of Genetics and Microbiology, Autonomous University of Barcelona, Spain.
Journal of Bacteriology
|January 1, 1991
Abstract:
We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.