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Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
Published on: November 4, 2015
ADSORPTION OF INFLUENZA VIRUS ON CELLS OF THE RESPIRATORY TRACT
1Laboratories of the International Health Division of The Rockefeller Foundation, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Influenza virus rapidly adsorbs to ferret lung cells, releasing infectious virus within hours. This interaction, similar to avian red blood cells, suggests receptor destruction is key to viral infection.
Area of Science:
- Virology
- Cell Biology
- Respiratory Medicine
Background:
- Influenza virus infects respiratory cells.
- Understanding virus-cell interactions is crucial for antiviral development.
Purpose of the Study:
- To investigate the interaction between influenza virus and ferret lung cells.
- To elucidate the mechanism of influenza virus adsorption and release.
Main Methods:
- Studied influenza virus and excised/perfused ferret lung cells.
- Inoculated virus intratracheally and observed adsorption and release.
- Assessed virus infectivity after heat or formalin treatment.
Main Results:
- Ferret lung cells rapidly adsorbed influenza virus.
- Pulmonary cells released adsorbed virus, with Influenza B showing 75% release in 5 hours.
- Released cells were incapable of further adsorption; inactivated virus was not released.
Conclusions:
- Influenza virus-lung cell interaction mirrors avian red blood cell interactions.
- In vivo, virus fixation increases over time, hindering release.
- Viral destruction of cell receptors may be essential for influenza parasitism.
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