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Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
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THE CONSTITUTION OF MITOCHONDRIA AND MICROSOMES, AND THE DISTRIBUTION OF NUCLEIC ACID IN THE CYTOPLASM OF A LEUKEMIC
1Laboratories of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
This study fractionated rat leukemic cell cytoplasm, finding ribose nucleic acid primarily associated with large components like mitochondria and microsomes, not free or in low molecular weight forms.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Cytoplasmic constituents of leukemic cells are complex.
- Understanding the distribution of nucleic acids within these cells is crucial.
Purpose of the Study:
- To fractionate rat leukemic cell cytoplasm.
- To determine the localization of ribose nucleic acid (RNA).
Main Methods:
- Differential centrifugation of rat tumor extracts.
- Analysis of elemental composition (nitrogen, phosphorus, lipids).
- Ribose nucleic acid and light absorption tests.
Main Results:
- Mitochondria isolated, showing high phospholipid content.
- Microsomes separated, with high phosphorus content linked to basophilia.
- RNA was not found free or in low molecular weight nucleoproteins.
Conclusions:
- Ribose nucleic acid in leukemic cell cytoplasm is associated with large structures.
- RNA is primarily localized within mitochondria and microsomes.
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