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THE SEDIMENTATION RATE OF THE BIOLOGICAL ACTIVITIES OF INFLUENZA A VIRUS

M A Lauffer1, G L Miller

  • 1Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, New Jersey.

The Journal of Experimental Medicine
|October 30, 2009
PubMed

Insights

Influenza A virus infectivity and red blood cell agglutination are linked to 100 mm particles. These findings support the association of biological activities with specific influenza virus components.

Area of Science:

  • Virology
  • Biophysics

Background:

  • Influenza A virus exhibits multiple biological activities, including infectivity and hemagglutination.
  • Understanding the physical properties of influenza virus is crucial for characterizing its infectious agents.

Purpose of the Study:

  • To determine the sedimentation rates of mouse infectivity principles for PR8 and F12 influenza A virus strains.
  • To correlate biological activities (infectivity and hemagglutination) with specific influenza virus components.

Main Methods:

  • Sedimentation rate analysis using a separation cell.
  • Assay of mouse infectivity, chicken red blood cell agglutination, and chick embryo infectivity.

Main Results:

  • Sedimentation rates of mouse infectivity principles were comparable to principal components of purified influenza A virus.
  • The majority of hemagglutinating activity and chick embryo infectivity sedimented at the same rate as the 100 mm component.
  • Some biological activity was associated with aggregated influenza virus particles.

Conclusions:

  • The study supports the association of mouse infectivity, hemagglutination, and chick embryo infectivity with the 100 mm spherical particles of influenza A virus.

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