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PLASMA PROTEIN PRODUCTION INFLUENCED BY AMINO ACID MIXTURES AND LACK OF ESSENTIAL AMINO ACIDS : A DEFICIENCY STATE
S C Madden1, F W Anderson, J C Donovan
1Department of Pathology, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Controlled nitrogen intake in dogs can maintain plasma protein production during hypoproteinemia. Oral amino acid mixtures are best utilized, supporting protein synthesis and reducing nitrogen loss.
Area of Science:
- Biochemistry
- Animal Nutrition
- Physiology
Background:
- Plasmapheresis in dogs leads to hypoproteinemia and altered protein metabolism.
- Controlled nitrogen intake is crucial for maintaining a steady state of plasma protein production.
Purpose of the Study:
- To investigate the impact of different nitrogen sources and administration routes on plasma protein production in hypoproteinemic dogs.
- To determine the essential amino acid requirements for maintaining protein synthesis and nitrogen balance.
Main Methods:
- Dogs underwent plasmapheresis to induce hypoproteinemia.
- Dietary nitrogen was supplied via controlled intravenous, subcutaneous, or oral administration of essential amino acid mixtures.
- Nitrogen balance and plasma protein levels were monitored.
Main Results:
- Oral administration of essential amino acids and glycine resulted in optimal utilization and lower urinary nitrogen excretion.
- Intravenous and subcutaneous routes showed good, but less efficient, plasma protein production.
- Specific amino acid deficiencies (leucine, tryptophan) severely impacted nitrogen balance and protein synthesis, while histidine deficiency increased urinary nitrogen.
Conclusions:
- Dietary amino acid composition and route of administration significantly influence plasma protein production and nitrogen utilization in dogs.
- Optimal amino acid mixtures are vital for maintaining protein homeostasis during induced hypoproteinemia.
- Further research is needed to identify unidentified factors in liver and yeast that may support protein metabolism.
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