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THE PREPARATION OF SECTIONS OF GUINEA PIG LIVER FOR ELECTRON MICROSCOPY
1Laboratories of The Rockefeller Institute for Medical Research, and The Research Laboratories, Interchemical Corporation, New York.
Abstract:
1. A method is described whereby sections of guinea pig liver cells can be prepared for electron microscopy after fixation. 2. The high resolving powerof the electron microscope reveals the presence of two components, one particulate, the other apparently of fibrous texture, in the ground substance of the cells.
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Preparation of Samples for Electron Microscopy
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...