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LOCALIZATION OF RIBONUCLEIC ACID IN THE CYTOPLASM OF LIVER CELLS.
The Journal of Experimental Medicine
|October 30, 2009
Summary
The basophilic material in rat liver cells, particularly during butter yellow-induced hyperplasia, is identified as ribonucleic acid (RNA). This finding is crucial for understanding chemical carcinogenesis and liver cell changes.
Area of Science:
- Biochemistry
- Cell Biology
- Toxicology
Background:
- Basophilic material in liver cell cytoplasm is observed during hyperplasia induced by the azo dye butter yellow.
- This material accumulates in hyperplastic liver cells and bile ducts, preceding tumor formation (hepatomas and cholangiomas).
Purpose of the Study:
- To characterize the chemical nature of the basophilic material in normal and hyperplastic rat liver cells.
- To investigate the role of this substance in chemically induced liver carcinogenesis.
Main Methods:
- Spectrophotometric analysis of ultraviolet absorption at 2537 Å.
- Feulgen reaction test to differentiate from deoxyribonucleic acid.
- Enzymatic digestion with ribonuclease, with and without lanthanum acetate protection.
Main Results:
- The basophilic material exhibits characteristics of ribonucleic acid (RNA).
- It absorbs UV radiation at 2537 Å and is Feulgen-negative.
- Ribonuclease effectively removes the material, which is protected by lanthanum acetate.
Conclusions:
- The basophilic substance accumulating in butter yellow-induced liver hyperplasia is confirmed to be RNA.
- This identification provides insight into the biochemical changes during chemical carcinogenesis in the liver.
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