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Updated: Jun 19, 2026

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology
Published on: October 3, 2016
THE CONSTRUCTION AND OPERATION OF EXPERIMENTAL ROOMS FOR THE STUDY OF AIR-BORNE INFECTION
O H Robertson1, T T Puck, H Wise
1Department of Medicine, The University of Chicago, Chicago.
Abstract:
This communication describes the construction and operation of two identical experimental rooms in which it is possible to produce and maintain a wide range of temperature and humidity with or without exchange of the room air. The ability to maintain a large air mass under constant conditions makes it possible to study the effects of different atmospheric states on air-suspended bacteria and viruses in relation to their survival, particle size, humidification, killing by lethal vapors, and host susceptibility. A brief description of the functioning of the rooms under experimental conditions is given.

