Related Experiment Video
Updated: Jun 19, 2026

04:41
Measuring Lactase Enzymatic Activity in the Teaching Lab
Published on: August 6, 2018
A MANOMETRIC METHOD FOR MEASURING THE ACTIVITY OF THE CL. WELCHII LECITHINASE AND A DESCRIPTION OF CERTAIN PROPERTIES
P C Zamecnik1, L E Brewster, F Lipmann
1Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, and the Biochemical Research Laboratory, Massachusetts General Hospital, Boston.
The Journal of Experimental Medicine
|October 30, 2009
Abstract:
A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.

