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Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
Published on: January 25, 2017
ELECTROPHORETIC STUDY OF ANTIVIRAL SERA
H Koprowski1, G Richmond, D H Moore
1Section of Viral and Rickettsial Research, Lederle Laboratories Division, American Cyanamid Company, Pearl River, and the Electrophoresis Laboratory, College of Physicians and Surgeons, Columbia University, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
This study investigated virus-neutralizing antibodies in animal sera. Researchers found that gamma-globulin fractions protected against Japanese B encephalitis and equine encephalomyelitis viruses, indicating its crucial role in immunity.
Area of Science:
- Immunology
- Virology
- Biochemistry
Background:
- Antibodies are crucial for viral immunity.
- Electrophoresis is a technique used to separate serum proteins.
Purpose of the Study:
- To identify the specific serum protein fractions responsible for neutralizing Japanese B encephalitis and equine encephalomyelitis viruses.
- To compare antibody localization in rabbit and chicken sera.
Main Methods:
- Animal sera (rabbit and chicken) were immunized against specific viruses.
- Sera were fractionated using electrophoresis.
- Fractions were tested for virus-neutralizing activity against infected mouse brain suspensions.
Main Results:
- Gamma-globulin fractions demonstrated protective and virus-neutralizing activity against all tested viruses.
- Japanese B encephalitis antibodies were exclusively in gamma-globulin.
- Venezuelan and Western equine encephalomyelitis antibodies were found in beta- and gamma-globulins.
- Chicken antibodies were associated with a specific fraction (component 4) with lower mobility.
Conclusions:
- Gamma-globulin is the primary carrier of neutralizing antibodies for Japanese B encephalitis and equine encephalomyelitis viruses.
- Antibody localization varies between species, with rabbits showing association with gamma-globulin and chickens with a specific fraction.
- Electrophoretic separation is effective in identifying the location of specific viral antibodies within serum.
Keywords:
VIRUSES/antiserum
