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STUDIES ON SPREADING FACTORS : II. THE EFFECT OF SERUM UPON HYALURONIDASE SPREADING ACTIVITY
1Worcester Foundation for Experimental Biology, Shrewsbury, and the Department of Physiology, Tufts Medical School, Boston.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Normal serum inhibits hyaluronidase in lab tests, but this antihyaluronidase activity is lost in skin. This suggests skin conditions prevent serum from blocking hyaluronidase effectively.
Area of Science:
- Biochemistry
- Immunology
- Dermatology
Background:
- Serum contains a factor that inhibits hyaluronidase.
- Hyaluronidase is an enzyme that can facilitate the spread of substances, including pathogens.
Purpose of the Study:
- To investigate the antihyaluronidase activity of serum under different conditions.
- To compare the in vitro and in vivo effectiveness of serum's inhibitory effect on hyaluronidase.
Main Methods:
- Studied the reaction between normal serum and hyaluronidase in vitro.
- Assessed hyaluronidase spreading activity in skin under in vivo conditions after incubation with serum.
Main Results:
- In vitro incubation showed serum possesses significant antihyaluronidase activity.
- In vivo incubation in skin revealed no inhibitory effect of serum on hyaluronidase spreading activity.
Conclusions:
- The ineffectiveness of serum in vivo suggests that skin environmental conditions are unfavorable for the serum-hyaluronidase inhibitory reaction.
- The disparity between in vitro and in vivo findings impacts understanding the role of this serum factor in defense mechanisms.
