PREPARATION OF HEMOGLOBIN SOLUTIONS FOR INTRAVENOUS INFUSION

Area of Science:

• Biochemistry
• Biomedical Engineering
• Hematology

Background:

• Plasma expanders are crucial in medical emergencies.
• Existing solutions face challenges with stability and purity.
• Oxyhemoglobin-based oxygen carriers offer potential but require robust preparation methods.

Purpose of the Study:

• To detail a procedure for preparing sterile, non-pyrogenic oxyhemoglobin solutions.
• To achieve plasma-like protein content and electrolyte composition.
• To ensure high oxygen-binding capacity and solution stability.

Main Methods:

• Developed a procedure for large-volume sterile oxyhemoglobin solution preparation.
• Implemented lipid removal techniques, targeting 95% reduction.
• Assessed hemoglobin activity and methemoglobin formation over time.

Main Results:

• Achieved sterile, non-pyrogenic oxyhemoglobin solutions with plasma-like composition.
• Solutions maintained 95-98% active hemoglobin capable of oxygen binding.
• 95% of total blood lipids were removed, and solutions were free of particulate matter.
• Stored solutions showed no methemoglobin formation for 2.5 months at 4°C, with minimal formation over 6 months.

Conclusions:

• The described procedure yields high-quality oxyhemoglobin solutions suitable for potential therapeutic use.
• The solutions demonstrate excellent stability and oxygen-carrying capacity.
• This method offers a scalable approach for producing advanced oxygen carriers.